Bitkisel Kaynaklı Fenolik Yapıdaki Bileşiklerin Olası Sitotoksik ve Genotoksik Etkilerinin Değerlendirilmesi

Abstract

Free oxygen radicals react with genetic material such as DNA, RNA and other cell macromolecules and the cause oxidative damage. There is a worldwide increasing interest about antioxidant compounds which could delay or prevent oxidative damage by inactivating free radicals. From the idea that nature is not harmful and studies about toxic effects of synthetic antioxidants showing that the usage of sources of natural antioxidants, vegetables, fruits, spices and teas are increasing. The phenolic compounds in plants are the main sources of antioxidant effects of herbs. In this thesis, antioxidant capacity, possible cytotoxic, genotoxic properties and protective effects of herbal phenolic compounds such as galangin, limonene, naringin, puerarin and ursolic acid have been investigated. Their antioxidant properties, possible cytotoxic effects, possible genotoxic effects and antigenotoxic effects against oxidative damage were investigated by trolox equivalent antioxidant capacity assay (TEAC), neutral red uptake assay (NRU) and 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) assay, single cell gel electrophoresis (COMET) and micronucleus assay (MN) method, respectively. In TEAC assay, antioxidant capacities of studied phenolic compounds were increased proportionately with concentration. In NRU and MTT assays, all of the studied compounds have showed cytotoxic effects at high concentrations in V79 cells. In COMET and MN assays, all of the studied compounds have not genotoxic effects at low concentrations and at concentrations below cytotoxicity they have decreased H2O2 induced oxidative damage.