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dc.contributor.authorTaban, B. Mercanoglu
dc.contributor.authorBen, U.
dc.contributor.authorAytac, S. A.
dc.date.accessioned2019-12-13T07:50:19Z
dc.date.available2019-12-13T07:50:19Z
dc.date.issued2009
dc.identifier.issn0022-0302
dc.identifier.urihttps://doi.org/10.3168/jds.2008-1537
dc.identifier.urihttp://hdl.handle.net/11655/18844
dc.description.abstractDuring the past few years, milk has presented a risk of Salmonella contamination; it has been implicated as the cause in several outbreaks of salmonellosis. Because conventional detection methods require 5 to 7 d for completion and involve several subcultivation stages followed by biochemical and serological tests, rapid and sensitive methods have been sought, mainly at the DNA level. Therefore, a study including milk samples was conducted to evaluate the performance of a combination of 2 techniques-immunomagnetic separation and polymerase chain reaction (PCR)-for the detection of Salmonella. The 16-, 14-, 12-, 10-, and 8-h nonselective pre-enrichment steps before immunomagnetic separation and the high-pure DNA preparation method before PCR were used in a combined assay. Milk samples, which were found to be Salmonella-negative by a reference method, were first inoculated with Salmonella Enteritidis. Next, the shortest preenrichment time that is required for detection of 1 or 10 cfu of Salmonella/mL by combined immunomagnetic separation-PCR assay was found by using 16-, 14-, 12-, 10-, and 8-h incubation periods. The detection limit using a 16-, 14-, or 12- h nonselective pre-enrichment was 1 to 10 cfu/mL. However, the sensitivity decreased to 10(1) and 10(2) cfu/mL, respectively, when 10- and 8-h pre-enrichments were used. This assay, in conjunction with a 12-h pre-enrichment, proved to be rapid (overall 16 h) and sensitive (1-10 cfu/mL) for the detection of Salmonella in milk samples and promising for routine use in the detection of Salmonella in milk.
dc.language.isoen
dc.publisherElsevier Science Inc
dc.relation.isversionof10.3168/jds.2008-1537
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectAgriculture
dc.subjectFood Science & Technology
dc.titleRapid Detection of Salmonella in Milk by Combined Immunomagnetic Separation-Polymerase Chain Reaction Assay
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.relation.journalJournal Of Dairy Science
dc.contributor.departmentGıda Mühendisliği
dc.identifier.volume92
dc.identifier.issue6
dc.identifier.startpage2382
dc.identifier.endpage2388
dc.indexingWoS
dc.indexingScopus


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