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dc.contributor.authorTaban, Birce Mercanoglu
dc.contributor.authorAytac, Sait Aykut
dc.contributor.authorAkkoc, Nefise
dc.contributor.authorAkcelik, Mustafa
dc.date.accessioned2019-12-13T07:50:23Z
dc.date.available2019-12-13T07:50:23Z
dc.date.issued2013
dc.identifier.issn1517-8382
dc.identifier.urihttps://doi.org/10.1590/S1517-83822013005000047
dc.identifier.urihttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3833132/
dc.identifier.urihttp://hdl.handle.net/11655/18855
dc.description.abstractIn the last decade, ready-to-eat (RTE) salad vegetables are gaining increasing importance in human diet. However, since they are consumed fresh, inadequate washing during processing can bring on some foodborne illnesses, like salmonellosis, since these food items have natural contamination from soil and water. During 2009–2010, a total of 81 samples were purchased arbitrarily from local markets in Ankara, and were examined for Salmonella contamination. Salmonella screening was performed by using anti-Salmonella magnetic beads system and polymerase chain reaction (PCR) identification of the suspected colonies. Then, the antibiotic resistance profiles of four Salmonella strains identified (strains RTE-1, RTE-2, RTE-3, and RTE-4) were also investigated, since the mechanism by which Salmonella spp. have accumulated antibiotic resistance genes is of interest. All strains showed resistance against sulfonamides (MIC > 128 mg/L). Further results suggested that associated sulfonamide resistance genes were encoded by the 55.0 kb plasmid of strain RTE-1 that involves no integrons. As a result of using two primers (P1254 and P1283) in randomly amplified polymorphic DNA-PCR (RAPD-PCR) analysis, two common amplicons (364 bp and 1065 bp) were determined. The findings of this study provide support to the adoption of guidelines for the prudent use of antibiotics in order to reduce the number of pathogens present on vegetable and fruit farms. Besides, since it is shown that these bacteria started to gain resistance to antibiotics, it is necessary to further investigate the prevalence of them in foods.
dc.relation.isversionof10.1590/S1517-83822013005000047
dc.rightsinfo:eu-repo/semantics/openAccess
dc.titleCharacterization Of Antibiotic Resistance In Salmonella Enterica Isolates Determined From Ready-To-Eat (Rte) Salad Vegetables
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.relation.journalBrazilian Journal of Microbiology
dc.contributor.departmentGıda Mühendisliği
dc.identifier.volume44
dc.identifier.issue2
dc.identifier.startpage385
dc.identifier.endpage391
dc.indexingPubMed
dc.indexingWoS
dc.indexingScopus


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