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dc.contributor.authorCakmak, Anil S.
dc.contributor.authorCakmak, Soner
dc.contributor.authorWhite, James D.
dc.contributor.authorRaja, Waseem K.
dc.contributor.authorKim, Kyungsook
dc.contributor.authorYigit, Sezin
dc.contributor.authorKaplan, David L.
dc.contributor.authorGumusderelioglu, Menemse
dc.date.accessioned2019-12-13T11:04:52Z
dc.date.available2019-12-13T11:04:52Z
dc.date.issued2016
dc.identifier.issn0736-0266
dc.identifier.urihttps://doi.org/10.1002/jor.23059
dc.identifier.urihttp://hdl.handle.net/11655/18972
dc.description.abstractBioelectrical regulation of bone fracture healing is important for many cellular events such as proliferation, migration, and differentiation. The aim of this study was to investigate the osteogenic differentiation potential of human mesenchymal stem cells (hMSCs) cultivated on silk scaffolds in response to different modes of electrostimulation (e.g., exogeneous and/or endogeneous). Endogeneous electrophysiology was altered through the use of monensin (10nM) and glibenclamide (10M), along with external electrostimulation (60kHz; 100-500mV). Monensin enhanced the expression of early osteogenic markers such as alkaline phosphatase (ALP) and runt-related transcription factor 2 (RUNX-2). When exogeneous electrostimulation was combined with glibenclamide, more mature osteogenic marker upregulation based on bone sialoprotein expression (BSP) and mineralization was found. These results suggest the potential to exploit both exogeneous and endogeneous biophysical control of cell functions towards tissue-specific goals. (c) 2015 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 34:581-590, 2016.
dc.language.isoen
dc.publisherWiley
dc.relation.isversionof10.1002/jor.23059
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectOrthopedics
dc.titleSynergistic Effect of Exogeneous and Endogeneous Electrostimulation on Osteogenic Differentiation of Human Mesenchymal Stem Cells Seeded on Silk Scaffolds
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.relation.journalJournal Of Orthopaedic Research
dc.contributor.departmentKimya Mühendisliği
dc.identifier.volume34
dc.identifier.issue4
dc.identifier.startpage581
dc.identifier.endpage590
dc.indexingWoS


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