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dc.contributor.authorCetinkaya, Gaye
dc.contributor.authorKahraman, Anıl Sera
dc.contributor.authorGümüşderelioğlu, Menemse
dc.contributor.authorArat, Sezen
dc.contributor.authorOnur, Mehmet Ali
dc.date.accessioned2019-12-13T11:04:56Z
dc.date.available2019-12-13T11:04:56Z
dc.date.issued2011
dc.identifier.issn0920-9069
dc.identifier.urihttps://doi.org/10.1007/s10616-011-9380-7
dc.identifier.urihttp://hdl.handle.net/11655/18979
dc.description.abstractFetal chondrocytes (FCs) have recently been identified as an alternative cell source for cartilage tissue engineering applications because of their partially chondrogenically differentiated phenotype and developmental plasticity. In this study, chondrocytes derived from fetal bovine cartilage were characterized and then cultured on commercially available Cytodex-1 and Biosilon microcarriers and thermosensitive poly(hydroxyethylmethacrylate)-poly(N-isopropylacrylamide) (PHEMA-PNIPAAm) beads produced by us. Growth kinetics of FCs were estimated by means of specific growth rate and metabolic activity assay. Cell detachment from thermosensitive microcarriers was induced by cold treatment at 4 degrees C for 20 min or enzymatic treatment was applied for the detachment of cells from Cytodex-1 and Biosilon. Although attachment efficiency and proliferation of FCs on PHEMA-PNIPAAm beads were lower than that of commercial Cytodex-1 and Biosilon microcarriers, these beads also supported growth of FCs. Detached cells from thermosensitive beads by cold induction exhibited a normal proliferative activity. Our results indicated that Cytodex-1 microcarrier was the most suitable material for the production of FCs in high capacity, however, 'thermosensitive microcarrier model' could be considered as an attractive solution to the process scale up for cartilage tissue engineering by improving surface characteristics of PHEMA-PNIPAAm beads.
dc.language.isoen
dc.publisherSpringer
dc.relation.isversionof10.1007/s10616-011-9380-7
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectBiotechnology & Applied Microbiology
dc.subjectCell Biology
dc.titleDerivation, Characterization And Expansion Of Fetal Chondrocytes On Different Microcarriers
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.relation.journalCytotechnology
dc.contributor.departmentKimya Mühendisliği
dc.identifier.volume63
dc.identifier.issue6
dc.identifier.startpage633
dc.identifier.endpage643
dc.indexingWoS


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