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dc.contributor.authorKalburcu, Tulden
dc.contributor.authorTuzmen, Munire Nalan
dc.contributor.authorAkgol, Sinan
dc.contributor.authorDenizli, Adil
dc.date.accessioned2019-12-16T09:19:09Z
dc.date.available2019-12-16T09:19:09Z
dc.date.issued2014
dc.identifier.issn1300-0527
dc.identifier.urihttps://doi.org/10.3906/kim-1301-87
dc.identifier.urihttp://hdl.handle.net/11655/19627
dc.description.abstractImmobilized metal chelate affinity chromatography (IMAC) support was practiced for alpha-amylase immobilization. Poly(hydroxyethylmethacrylate-methacryloylamidotryptophan)-Ni2+ [p(HEMA-MAT)-Ni2+] nanospheres, average diameter 100 nm, were produced by surfactant free emulsion polymerization. Characterizations of p(HEMA-MAT)-Ni2+ nanospheres were carried out by Fourier transform infrared (FTIR) spectroscopy and scanning electron microscope (SEM). In addition, average particle size, size distribution, and surface charge were specified. The amount of N-methacryloylamidotryptophan (MAT) incorporated to polymer was determined as 1.95 mmol/g polymers by using nitrogen stoichiometry. The specific surface areas of poly(hydroxyethylmethacrylate) [p(HEMA)] and p(HEMA-MAT) nanospheres were calculated as 1856 m(2)/g and 1914 m(2)/g, respectively. Protein adsorption increased with increasing initial protein concentration and maximum alpha-amylase adsorption on p(HEMA-MAT)-Ni2+ nanospheres was observed at pH 4.0. Both free and immobilized alpha-amylase showed pH optimum at pH 7.0. It was determined that the immobilized alpha-amylase had better thermostability than the free one. Immobilization of the enzyme did not significantly change the kinetic parameters. The storage stability of alpha-amylase increased upon immobilization. It was also observed that p(HEMA-MAT)-Ni2+ nanospheres can be repeatedly used for alpha-amylase immobilization.
dc.language.isoen
dc.publisherScientific Technical Research Council Turkey-Tubitak
dc.relation.isversionof10.3906/kim-1301-87
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectChemistry
dc.subjectEngineering
dc.titleImmobilized Metal Ion Affinity Nanospheres For Alpha-Amylase Immobilization
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.relation.journalTurkish Journal Of Chemistry
dc.contributor.departmentKimya
dc.identifier.volume38
dc.identifier.issue1
dc.identifier.startpage28
dc.identifier.endpage40
dc.indexingWoS
dc.indexingScopus


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