In Vitro Effects Of Rosmarinic Acid On Glutathione Reductase And Glucose 6-Phosphate Dehydrogenase
Demirezer, L. Omur
Ulusu, N. Nuray
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Context: Glutathione reductase (GR, NADPH:oxidized glutathione oxidoreductase, E. C 22.214.171.124) is a flavoprotein that catalyzes the NADPH-dependent reduction of oxidized glutathione (GSSG). GR is a crucial enzyme in the antioxidant system by maintaining reduced glutathione (GSH). Glucose 6-phosphate dehydrogenase (G6PD, glucose 6-phosphate (G6P):NADP(+) oxidoreductase, EC 126.96.36.199) is the key regulatory enzyme of the pentose phosphate pathway and maintains NADPH for reductive reactions. Objective: Rosmarinic acid (RA; alpha alpha-O-caffeoyl-3,4-dihydroxyphenyl lactic acid) is an ester of caffeic acid (CA) and 3,4-dihydroxyphenyllactic acid. It has a number of interesting biological activities. The inhibiting activities of the RA on GR and G6PD are investigated here for the first time. Materials and methods: GR and G6PD were purified from tissues, then the effects of RA are investigated. Results: This study reports that RA, which was isolated from Echium vulgare L. (Boraginaceae), inhibits purified GR and G6PD in a concentration-dependent manner. Kinetic characterizations and inhibition constants are investigated. Discussion and conclusion: Because of their importance in the antioxidative defense system, investigation of the inhibitors of these enzymes is important for drug development.