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dc.contributor.authorErgünay, Koray
dc.contributor.authorLitzba, N.
dc.contributor.authorLo, M.M.
dc.contributor.authorAydoǧan, S.
dc.contributor.authorSaygan, M.
dc.contributor.authorUs, D.
dc.contributor.authorWeidmann, M.
dc.contributor.authorNiedrig, M.
dc.date.accessioned2020-01-31T13:22:51Z
dc.date.available2020-01-31T13:22:51Z
dc.date.issued2011
dc.identifier.issn1557-7759
dc.identifier.urihttps://doi.org/10.1089/vbz.2010.0224
dc.identifier.urihttps://www.scopus.com/inward/record.url?eid=2-s2.0-79959483104&partnerID=40&md5=6375796ac4499966c0f5c826a80ec376
dc.identifier.urihttp://hdl.handle.net/11655/21978
dc.description.abstractIntroduction: Sandfly fever virus (SFV) serotypes sandfly fever Naples virus, sandfly fever Sicilian virus, and sandfly fever Cyprus virus cause febrile diseases, whereas Toscana virus (TOSV) is responsible for aseptic meningoencephalitis. Diagnosis and surveillance of TOSV depend heavily on virus serology, and various commercial assays utilizing various antigen sources and formats have been available. The aim of this study was to perform comparative evaluation of commercially available serological assays for anti-TOSV immunoglobulins. Materials and Methods: A collection of 120 sera from healthy blood donors from an endemic region, previously identified to be reactive for antibodies against various SFV serotypes by indirect immunofluorescence test (IIFT), was reevaluated for IgG/IgM via IIFT, enzyme-linked immunosorbent assay, and an immunoblot assay manufactured by Euroimmun, Diesse, and Mikrogen, respectively. Virus neutralization test (VNT) was performed for 99 sera using standard TOSV, sandfly fever Sicilian virus, and sandfly fever Naples virus strains. Results: A total of 89 samples (74.2%) were reactive for TOSV IgG in at least one of the commercial assays, and 31 samples (31.3%) were reactive in VNT for various SFV serotypes. Average percentage agreements among commercial assays and between VNT and the commercial assays were noted as 57.8% and 62.6%, respectively. No significant correlation between assay results and VNT titers was observed. SFV IgM antibodies were detected in a total of eight samples (6.7%) via IIFT, which were nonreactive in enzyme-linked immunosorbent assay and VNT. Discussion: Commercial diagnostic immunoassays displayed slight to fair agreement for TOSV IgG as assessed via kappa and percentage agreement values. The results could only be confirmed via virus neutralization in a portion of the samples, and overall agreement between the commercial assays and VNT was slight. Commercial assays such as immunoblot can be used in addition to VNT for confirmation of TOSV exposure.tr_TR
dc.language.isoentr_TR
dc.publisherMary Ann Lieberttr_TR
dc.relation.isversionof10.1089/vbz.2010.0224tr_TR
dc.rightsinfo:eu-repo/semantics/openAccesstr_TR
dc.subjectDiagnosistr_TR
dc.subjectSandfly fever virustr_TR
dc.subjectSerologytr_TR
dc.subjectToscana virustr_TR
dc.subject.lcshTıptr_TR
dc.titlePerformance of Various Commercial Assays for the Detection of Toscana Virus Antibodiestr_TR
dc.typeinfo:eu-repo/semantics/articletr_TR
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.relation.journalVector-Borne and Zoonotic Diseasestr_TR
dc.contributor.departmentTıbbi Mikrobiyolojitr_TR
dc.identifier.volume11tr_TR
dc.identifier.issue6tr_TR
dc.identifier.startpage781tr_TR
dc.identifier.endpage787tr_TR
dc.indexingScopustr_TR
dc.fundingYoktr_TR


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