Show simple item record

dc.contributor.authorAksoy, Ceren
dc.contributor.authorAerts Kaya, Fatima
dc.contributor.authorKuşkonmaz, Bülent Barış
dc.contributor.authorUçkan, Duygu
dc.contributor.authorSevercan, Feride
dc.date.accessioned2020-02-19T10:28:58Z
dc.date.available2020-02-19T10:28:58Z
dc.date.issued2014
dc.identifier.issn0161-9152
dc.identifier.urihttps://doi.org/10.1007/s11357-014-9691-7
dc.identifier.urihttp://hdl.handle.net/11655/22165
dc.description.abstractStem cell studies hold enormous potential for development of new therapies for tissue regeneration and repair. Bone marrow mesenchymal stem cells (BM-MSCs) can differentiate into a variety of nonhematopoietic tissues and contribute maintenance of healthy hematopoiesis by providing supportive cellular microenvironment into BM. Here, we investigated age-related differences in BM-MSCs by using attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy and FTIR imaging together with hierarchical clustering as a novel methods to clarify global alterations in the structure and function of macromolecules in characterized BM-MSCs of different aged donors. The results may contribute to identification of agerelated new molecular marker(s) to determine the effects of donor age on MSCs. The spectral results reflected that there were significant increases in the concentration of saturated lipids, proteins, glycogen, and nucleic acids in children and adolescent group BM-MSCs when compared to the infants and early and mid adults. The concentration of mentioned macromolecules in adult (early and mid) BM-MSCs were significantly lower than the concentrations in the children and adolescents. These results were attributed to the increase in the proliferation activity in younger BM-MSCs. The distribution of macromolecules into the cells was shown as in the form of chemical maps by FTIR imaging, and the results are in agreement with the ATR-FTIR spectroscopy results. The cellular activity degree was determined by the thiazolyl blue tetrazolium bromide (MTT) proliferation assay to support ATR-FTIR spectroscopy results. BM-MSCs of five different age groups were discriminated by making the hierarchical cluster analysis where the spectral data according to alterations in structure and composition of macromolecules were considered.tr_TR
dc.language.isoentr_TR
dc.publisherSpringertr_TR
dc.relation.isversionof10.1007/s11357-014-9691-7tr_TR
dc.rightsinfo:eu-repo/semantics/openAccesstr_TR
dc.subjectATR-FTIR spectroscopytr_TR
dc.subjectFTIR imagingtr_TR
dc.subjectBone marrow mesenchymal stem cellstr_TR
dc.subjectStem cell agingtr_TR
dc.subjectDonor age effecttr_TR
dc.subject.lcshR/W - Tıptr_TR
dc.titleStructural Investigation Of Donor Age Effect On Human Bone Marrow Mesenchymal Stem Cells: Ftir Spectroscopy And Imagingtr_TR
dc.typeinfo:eu-repo/semantics/articletr_TR
dc.typeinfo:eu-repo/semantics/publishedVersiontr_TR
dc.relation.journalAgetr_TR
dc.contributor.departmentKök Hücre Bilimleritr_TR
dc.identifier.volume36tr_TR
dc.identifier.issue4tr_TR
dc.identifier.startpage9691tr_TR
dc.identifier.endpage9707tr_TR
dc.indexingWoStr_TR
dc.fundingYoktr_TR


Files in this item

This item appears in the following Collection(s)

Show simple item record