Kuantum Noktalarının Memeli Hücrelerindeki Sitotoksik ve Genotoksik Etkileri
Özkan Vardar, Deniz
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In recent years, because of the increase in the technologies and the uses of the nanomaterials, toxicology has been the subject of many researches. Despite the potential advantages of the nanoparticles (NPs), it has been suggested to trigger undesirable hazardous interactions with biological systems generating harmful effects. Therefore, in the society, there is an increasing concern about the potential effects on human health and enviromental effects due to the exposure of NPs. Many of the NPs have been reported to induce adverse effect such as oxidative stress, cytotoxicity, DNA damage, inflammation, which are known to be crucial for the development of many diseases. Thus it is crucial to ensure safety use of new nanomaterial technologies to avoid undersirable effects. In this thesis, it was aimed to evaluate the cytotoxic and genotoxic effects of quantum dots which have been aimed to be used in many areas such as bioimaging, diagnosis and mechanics. Quantum dots have been used in solar batteries and composites that consist of small particles with arrangeble energy levels. In this study, the cytotoxicity of silver sulfides of 2-mercaptopropionic acid (2-MPA) and meso-2,3-dimercaptosuccinic acid (DMSA) quantum dots were evaluated by neutral red uptake (NRU) in Chinese hamster fibroblast cell lines (V79). The genotoxicity of these quantum dots were assassed by single cell electrophoresis method (COMET). Epigenetic changes were also examined by real time protein chain reaction (RT-PCR) technique.