Donepezil Yüklü Nanopartiküler İlaç Taşıyıcı Sistemlerin Hazırlanması ve In Vitro Değerlendirilmesi
Özet
There are about 46 million dementia worldwide today. The cause of disease is
unknown and there is currently no treatment to stop or slow the degenerative
process.1
However, there are some symptomatic treatment approaches to
partially improve the cognitive functions of Alzheimer’ s Disease, to control
emotional and psycologycal changes, and to improve the quality of life of patients
with clinical practice. 2 Donepezil is a reversible cholinesterase inhibitor used in the
treatment of Alzheimer’ s Disease. The cholinergic system plays an important role
in learning, memory, and severe memory impairment due to cholinergic
dysfunctions in Alzheimer’ s Disease.
3 New strategies are needed, such as
osmotically opening the blood brain barrier or devoloping drug delivery systems, in
order for drug molecule to bypass the blood brain barrier because of the
inadequacy of traditional transport mechanisms. Interventional techniques that
bypass blood brain barrier include the use of drug carrying nanoparticle systems
and intranasal pathway.1 Polymeric nanoparticles protect nanoparticles in vitro and
in vivo degradation, release drugs in a controlled manner, and offer the possibility
of drug targeting.4
In this study, PLGA nanoparticles loaded with Donepezil agent were prepared by
solvent evaporation technique. Several techniques have been tried for preparation
of nanoparticles and the activity of these techniques in preparing nanoparticles
has been observed. Parameters affecting the particle size were investigated and
reported in the optimal technique for preparing Donepezil active substance loaded
PLGA nanoparticles. For the characterization of the prepared nanoparticles,
HPLC, FTIR, particle size measurements were performed, particle preparation
efficiency, encapsulation efficiency and loading rates were calculated.
Furthermore, the analytical method has been developed and its validity proved to
be able to determine the Donepezil drug substance by the HPLC method.
