Kitosan Nanopartiküllerine Ve Kitosan-Halloysit Nanokompozitlerine Metronidazole Yüklenmesi, Salınımı Ve Adsorpsiyonunun İncelenmesi
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In this thesis study, it was aimed to obtain the drug carrier and adsorbent material with superior properties than the two components by making the nanoparticles of chitosan nanocomposite by using ionic gelation technique and making it nanocomposite with halloysite nanotubes. Chitosan-Halloysite nanocomposites were loaded with the Metronidazole antibiotic selected from the nitroimidazole family. Characterization of chitosan-halloysite nanocomposites was performed, and the morphology and particle size range of the hybrid nanotubes were determined. The adsorption of Metronidazole to chitosan nanoparticles and chitosan-halloysite nanocomposites were investigated, and the adsorption capacities and efficiencies of the particulates were compared. Then Metronidazole release was examined from chitosan nanoparticles and chitosan-halloysite nanocomposites. The effects of chitosan / TPP ratio on the sizes of chitosan nanoparticles synthesized by ionic gelation technique have been investigated. The analyzes show that the smallest nanoparticles are obtained at pH = 5 and 0.25% of Tripolyphosphate as the crosslinker. The effect of parameters such as pH, composite ratio, drug concentration and particle size on drug adsorption of Chitosan and Halloysite-Chitosan nanocomposite were investigated. At optimal conditions in chitosan nanoparticle, the adsorption efficiency of the particles was 33.45 % at the end of the 3rd hour, while the nanocomposite was found to be 66.14 %. Phosphate Buffer and Hydrochloric Acid buffers were used to form the in-vivo medium in order to have metronidazole release values from the particles. The encapsulation efficiency (% EE), loading capacities and percentages of release of the particles were calculated. Scanning electron microscopy (SEM), transmission electron microscopy (TEM) and Brunauer-Emmett-Teller (BET) instruments were used to determine the morphology of the chitosan and chitosan-halloysite hybrid nanotubes. The particle size range was measured using a zetasizer. By using Fourier Transform Infrared Spectroscopy (FTIR), molecular bond characterization is performed; the functional groups in the structure of the particles, whether the two compounds are the same, the state of the bonds in the structure, the binding sites are determined. Quantities of Metronidazole remaining after adsorption of the liquid solution were determined using UV spectrophotometer. In order to find out how the temperature change affects the particles, they were analyzed by Thermogravimetric (TGA) and Differential Scanning Calorimetry (DSC) instruments.