Piknogenol ve Kurkuminin Çeşitli Kanser Hücre Hatlarında Sisplatin Sitotoksisitesine Etkilerinin İncelenmesi
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Pycnogenol from Pinus pinaster and curcumin from Curcuma longa L., are poliphenolic compounds and commonly used in many diseases traditionally due to their high antioxidant properties. Cisplatin, an antineoplastic agent, is commonly used in the treatment of solid tumors such as testis, over, bladder, prostate, cervix, and lung cancer. In the treatment of cancer, it is aimed to increase anticancer effect and decrease cytotoxicity using various plant-derived phenolic compounds with chemotherapeutic drugs. Since the studies on the interrractions of pycnogenol and curcumin with anticancer drugs are insufficient, in this thesis, it was aimed to determine the cytotoxic effects of these phenolic compounds in Chinese hamster lung fibroblast (V79), human cervix adenocarcinoma (HeLa), and human hepatocellular carcinoma (HepG2) cells by MTT assay, to evaluate the cell viability in combination with cisplatin, and to clarfy the anticancer affect of cisplatin. Pycnogenol decreased IC50 of cisplatin in V79, HeLa, and HepG2 cells, at the concentrations of 500 μM, 15.6-500 μM and 125-500 μM, respectively for 24 h incubation. Pycnogenol decreased IC50 of cisplatin at lower concentrations in HepG2 and V79 cells and at higher concentrations in HeLa cells for 48 h incubation. Curcumin decreased IC50 of cisplatin in V79, HeLa, and HepG2 cells, at the concentrations of 500 μM, 500 μM, and 250-500 μM, respectively for 24 h incubation. Curcumin decreased IC50 of cisplatin at lower concentrations in all studied cells for 48 h incubation. In conclusion, our findings may show that pycnogenol and curcumin having different cytotoxic profiles according to cell types and may play a role in cervical and hepatic cancer cell growth and progression, however, further in vitro and in vivo studies are required to confirm these effects and to determine their interactions with anticancer drugs.