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Polyproline Tetramer Organizing Peptides in Fetal Bovine Serum Acetylcholinesterase

Biberoglu, Kevser; Schopfer, Lawrence M.; Saxena, Ashima; Tacal, Ozden; Lockridge, Oksana (Elsevier Science Bv, 2013)

Acetylcholinesterase (AChE) in the serum of fetal cow is a tetramer. The related enzyme, butyrylcholinesterase (BChE), in the sera of humans and horse requires polyproline peptides for assembly into tetramers. Our goal was ...

The Proline-Rich Tetramerization Peptides In Equine Serum Butyrylcholinesterase

Biberoglu, Kevser; Schopfer, Lawrence M.; Tacal, Ozden; Lockridge, Oksana (Wiley-Blackwell, 2012)

Soluble, tetrameric, plasma butyrylcholinesterase from horse has previously been shown to include a non-covalently attached polyproline peptide in its structure. The polyproline peptide matched the polyproline-rich region ...

Monoclonal Antibody That Recognizes Diethoxyphosphotyrosine-Modified Proteins And Peptides Independent Of Surrounding Amino Acids

Onder, Seda; Dafferner, Alicia J.; Schopfer, Lawrence M.; Xiao, Gaoping; Yerramalla, Udaya; Tacal, Ozden; Blake, Thomas A.; Johnson, Rudolph C.; Lockridge, Oksana (Amer Chemical Soc, 2017)

Acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) are irreversibly inhibited by organophosphorus pesticides through formation of a covalent bond with the active site serine. Proteins that have no active site ...

Hupresin Retains Binding Capacity For Butyrylcholinesterase And Acetylcholinesterase After Sanitation With Sodium Hydroxide

Onder, Seda; David, Emilie; Tacal, Ozden; Schopfer, Lawrence M.; Lockridge, Oksana (2017)

Hupresin is a new affinity resin that binds butyrylcholinesterase (BChE) in human plasma and acetylcholinesterase (AChE) solubilized from red blood cells (RBC). Hupresin is available from the CHEMFORASE company. BChE in ...

Delipidation Of Plasma Has Minimal Effects On Human Butyrylcholinesterase

Onder, Seda; Tacal, Ozden; Lockridge, Oksana (2018)

Human butyrylcholinesterase (BChE) is purified in large quantities from Cohn fraction IV-4 to use for protection against the toxicity of chemical warfare agents. Small scale preliminary experiments use outdated plasma from ...

Methamidophos, Dichlorvos, O-Methoate And Diazinon Pesticides Used In Turkey Make A Covalent Bond With Butyrylcholinesterase Detected By Mass Spectrometry

Tacal, Ozden; Lockridge, Oksana (Wiley, 2010)

Organophosphorus pesticides used most commonly in Turkey include methamidophos, dichlorvos, O-methoate and diazinon. These toxic chemicals or their metabolites make a covalent bond with the active site serine of ...

Mass Spectral Detection of Diethoxyphospho-Tyrosine Adducts on Proteins from Hek293 Cells Using Monoclonal Antibody Depy for Enrichment

Onder, Seda; Schopfer, Lawrence M.; Tacal, Ozden; Blake, Thomas A.; Johnson, Rudolph C.; Lockridge, Oksana (2018)

Use Of Hupresin To Capture Red Blood Cell Acetylcholinesterase For Detection Of Soman Exposure

Onder, Seda; Schopfer, Lawrence M.; Cashman, John R.; Tacal, Ozden; Johnson, Rudolph C.; Blake, Thomas K.; Lockridge, Oksana (Amer Chemical Soc, 2018)

Toxicity from acute exposure to nerve agents and organophosphorus toxicants is due to irreversible inhibition of acetylcholinesterase (AChE) in the nervous system. AChE in red blood cells is a surrogate for AChE in the ...