REAKTİF BOYALARIN MİKROORGANİZMALAR KULLANILARAK GİDERİLMESİ
İĞDE, ZEYNEP KEVSER
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Industrial wastewater causes serious environmental pollution in many parts of the world. Synthetic azo dyes, one of the pollutants causing this, are widely used in the textile industry. Azo dyes are difficult molecules to degrade due to their complex structures. In recent years, many studies have been carried out using microorganisms for biosorption and biodegradation of dye-containing wastewaters. The biological remediation of the dyes is economical, effective and harmless compared to other remediation methods.The biological remediation of the dyes is economical, effective and harmless compared to other treatment methods. In this study, the color removal of Reactive Black 5 (RB-5), Reactive Green 19 (RG-19), Reactive Brown 10 (RB-10) and Solochrome Black (SB) dyes was investigated using Phanerochaete chrysosporium and Chlorella vulgaris. The study was carried on with P. chrysosporium since no effective result was obtained from Chlorella vulgaris produced in the BBM (Bold Basal Medium) medium. It was obtained with P. chrysosporium at the Tien-Kirk feeder, Reactive Black 5, 92.5%, among the best color removal rate tested. Decolorization rates of other dyes were determined as 83% for RG-19, 63% for RB-10 and 63% for SB. For this reason, the experiment has been diluted with RB-5 and Tien-Kirk media. The effects of parameters such as dye concentration, pH, temperature, agitation speed, N and C sources on color removal were investigated. Color removal is measured spectrophotometrically. The obtained data were analyzed using SPSS 15 Software. As a result of the work done, Reactive Black 5, Reactive Brown and Solochrome Black dyes showed maximum color clearance at the end of the third day, while Reactive Green, maximum decolorization was observed at the end of the fifth day. Among the parameters tested in the optimization studies, the best decolorization was observed at a pH 4.5, a shaking speed of 150 rpm and a temperature of 30 oC. Among the tested carbon sources, the best color removal was observed in the nutrient-free medium, in starch-containing medium and in nitrogen-rich medium. According to the results obtained from this study, it is concluded that Phosphate RB-5 dye of Phanerochaete chrysosporium is very effective on color removal.