Şaşılık Hastalarında Aşırı Fonksiyon Gösteren Alt Oblik Kas Biyopisilerinde Klinikopatolojik Korelasyon ve Satellit Hücre Populasyonunun Belirlenmesi
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ABSTRACT Baytaroğlu A. Identification of satellite cell population and clinicopathologic correlation in overacting inferior oblique muscle specimens of strabismus patients. Hacettepe University Faculty of Medicine, Department of Ophthalmology, Thesis in Ophthalmology, Ankara, 2017. The purpose of this study is to immunohistochemically evaluate a stem cell niche, satellite cells, in different types of strabismus and to further investigate whether a clinicopathologic correlation exists between clinically measured degree of inferior oblique overaction and immunohistochemical parameters. Fifty-one patients who underwent strabismus surgery with inferior oblique myectomy for different underlying strabismus types were evaluated in this study. Patients were divided into two main groups in respect to their inferior oblique overaction mechanism. V pattern strabismus patients and infantile esotropia patients were evaluated as primary inferior oblique overaction. Congenital or acquired 4th nerve palsy patients were evaluated under the secondary inferior oblique overaction group. Immunohistochemical markers used for detecting satellite cell niche and active satellite cells were Pax7, NCAM and MyoD1 respectively. Difference in myofiber diameters, number of angular fibers, endomysial and perimysial fibrosis degree, and presence and degree of inflammation and vacuolisation were evaluated as histopathologic changes. Inferior oblique overaction was subjectively measured and scaled between 0-4. A statistically significant difference of all three immunohistochemical markers was observed between primary and secondary overacting group (p<0,001). Another significant finding between these two groups was higher angular fiber count and the degree of perimysial fibrosis in the latter group. But a correlation between immunohistochemical markers and the degree of inferior oblique overaction could not be demonstrated in this study. In conclusion, even though it does not correlate with clinical findings, active satellite cell population plays a role in the pathogenesis of different strabismus types. Key Words: Immunohistochemistry, inferior oblique muscle, MyoD, NCAM, Pax7, satellite cell, strabismus.