Çinko Oksit ve Bakır Oksit Nanoparçacıklarının in Vitro Sistemler Üzerindeki Toksik Etkilerinin Araştırılması

Abstract

Zinc oxide (ZnO) and copper oxide (CuO) NPs are commonly used metal nanoparticles with a high toxicity potential. However, information on toxicity mechanisms like other NPs, is limited. In this study, the possible cytotoxic effects of ZnO and CuO NPs on cell lines, either alone or in combination, on mouse fibroblast (L929) and human neuroblastoma (SH-SY5Y) cell lines were assessed and the protective effects of diethylenetriaminepentaacetic acid (DTPA), N-acetylcysteine and taurine have been examined. As a result of MTT and neutral red (NR) experiments, a time- and dose-dependent cytotoxic effect was observed with ZnO NPs, time-dependent cytotoxic effect with CuO NPs and concentration-dependent cytotoxic effect with mixture in L929 cells. Reactive oxygen species (ROS) and mitochondrial membrane potential (MMP) increased, apoptosis and intracellular calcium levels were found to be lower than control levels. CuO and mixture induced autophagy and in the presence of NAC and taurine, these levels were decreased and no increase in IL-6 production was observed. In SH-SY5Y cells, MTT and NK tests resulted in lower cytotoxicity than observed in L929 cell line. It was determined that ROS levels and MMP were elevated with the mixture. Apoptosis was induced, intracellular calcium levels was decreased with this NPs alone or in combination; IL-8 levels were not detected and no increase in IL-6 levels were observed.