Prenatal Bisfenol A ve/veya Di-2-Etil Hekzil Ftalat Maruziyetinin Erkek Üreme Sistemi Üzerine Etkilerinin İncelenmesi.
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Bisphenol A (BPA) and di (2-ethylhexyl) phthalate (DEHP) are chemical substances that are used in a wide variety of industrial products, with proven endocrine disrupting properties. Today, with the increasing use of these substances, it is possible that exposure to more than one substance at a time can occur. As prenatal and early postnatal periods are critical periods for development, exposure to endocrine disruptors in these periods are suggested to cause developmental defects of organs, reproductive problems and metabolic and hormonal disturbances by different mechanisms. In such periods, as the enzyme and defense mechanisms are not fully developed, the apprehension for the observation of adverse effects arises the need to perform research. In this thesis, the prenatal and early postnatal (lactation) exposure to BPA and DEHP together versus single exposures and as well as versus control group are evaluated and oxidative stress as one of the underlying mechanisms was commentated. Pregnant Sprague Dawley rats were divided into four groups as control, BPA, DEHP and BPA plus DEHP and dosed by oral gavage during pregnancy and lactation period. The male offspring of born from those rats were chosen randomly and their sperm parameters, plasma reproductive hormone levels, testicular histopathological and apoptotic changes were evaluated when they reach to adulthood (twelfth week) in order to evaluate the unwanted effects on reproductive system. In addition, oxidative stress parameters (lipid peroxidation, protein oxidation, glutathione levels, and oxidative DNA damage) were investigated in the testicular tissue. The results showed that fetal and neonatal exposure to endocrine disrupting chemicals can lead to significant histopathological and apoptotic alterations in testicular tissue, along with changes in sperm count, motility and morphology. Combined exposure particularly caused more significant effects. In BPA plus DEHP group, decreases in total glutathione levels and increases in lipid peroxidation vs. control group suggests that oxidative stress may be one of the possible underlying mechanisms for the adverse effects. All of the findings suggests that prenatal and early postnatal (lactation) exposures to BPA and/or DEHP can lead to undesired effects in adulthood of the male offspring.