Daimi Dişlerde Fissür Örtücü Uygulaması Sırasında Meydana Gelen Tükürük Kontaminasyonunun Mikrosızıntı Değerleri Üzerine Etkisinin İncelenmesi
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Simsek, H., Evaluation of the Effects of Saliva Contamination on the Microleakage Values of Permanent Teeth During the Application of Fissure Sealants, Hacettepe University Health Sciences Institute Specialty Thesis in Pediatric Dentistry, Ankara, 2019. This study aimed to evalaute the effect of different surface conditioning methods on the microleakage resistance of fissure sealants following saliva contamination. The study was carried out on 156 human third molars in 16 subgroups (2X4X2) under two main groups. 3M Clinpro™ Sealant (3M, St. Paul, Minnesota, ABD) was used in Group A, while Ultraseal XT Hydro (Ultradent, South Jordan, Utah, ABD) was used in Group B. The subgroups were formed with four different surface conditioning methods (laser etching, acid etching, etch-and-rinse adhesive system and self-etch adhesive system). In each subgroup, the conditioned occlusal enamel surfaces were contaminated with artificial saliva solution before fissure sealant application. After this step, in the half of the specimens (e.g. A1) the occlusal surfaces were washed, dried, and the respective fissure sealant was applied. In the other half (e.g. A2), however, the respective surface conditioning method was repeated and with the application of the respective fissure sealant the procedure was completed. After thermal cycling, the samples were stored in basic fuchsin solution, sectioned, photographed under a stereomicroscope and dye penetration was measured. Microleakage values observed in the study groups are A1=A2=A7>A3=A8>A4=A5=A6 and B1=B2=B7=B8>B3>B4=B5=B6 (p<0,05). Group A showed significantly less microleakage than Group B (p<0,05). The least microleakage was observed in subgroups A5, A6, B5 and B6 in which an etch-and-rinse adhesive system was applied. Highest microleakage values were observed in subgroups B7 and B8 where a self-etch adhesive was used. In both groups, laser etching after saliva contamination had no positive effect on microleakage resistance.