Bitkisel Kaynaklı Fenolik Yapıdaki Bileşiklerin Olası Sitotoksik ve Genotoksik Etkilerinin Değerlendirilmesi
Abstract
Free oxygen radicals
react with genetic material such as DNA, RNA and other cell macromolecules and
the cause oxidative damage. There is a worldwide increasing interest about
antioxidant compounds which could delay or prevent oxidative damage by
inactivating free radicals. From the idea that nature is not harmful and studies about
toxic effects of synthetic antioxidants showing that the usage of sources of natural
antioxidants, vegetables, fruits, spices and teas are increasing. The phenolic
compounds in plants are the main sources of antioxidant effects of herbs. In this
thesis, antioxidant capacity, possible cytotoxic, genotoxic properties and protective
effects of herbal phenolic compounds such as galangin, limonene, naringin, puerarin
and ursolic acid have been investigated. Their antioxidant properties, possible
cytotoxic effects, possible genotoxic effects and antigenotoxic effects against
oxidative damage were investigated by trolox equivalent antioxidant capacity assay
(TEAC), neutral red uptake assay (NRU) and 3-(4,5-dimethylthiazol-2-yl)-2,5-
diphenyltetrazolium bromide (MTT) assay, single cell gel electrophoresis (COMET)
and micronucleus assay (MN) method, respectively. In TEAC assay, antioxidant
capacities of studied phenolic compounds were increased proportionately with
concentration. In NRU and MTT assays, all of the studied compounds have showed
cytotoxic effects at high concentrations in V79 cells. In COMET and MN assays, all
of the studied compounds have not genotoxic effects at low concentrations and at
concentrations below cytotoxicity they have decreased H2O2 induced oxidative
damage.