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P-31 Nmr Correlation Maps Of O-18/O-16 Chemical Shift Isotopic Effects For Phosphometabolite Labeling Studies

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Date
2011
Author
Juranic, Nenad
Nemutlu, Emirhan
Zhang, Song
Dzeja, Petras
Terzic, Andre
Macura, Slobodan
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Abstract
Intramolecular correlations among the O-18-labels of metabolic oligophosphates, mapped by J-decoupled P-31 NMR 2D chemical shift correlation spectroscopy, impart stringent constraints to the O-18-isotope distributions over the whole oligophosphate moiety. The multiple deduced correlations of isotopic labels enable determination of site-specific fractional isotope enrichments and unravel the isotopologue statistics. This approach ensures accurate determination of O-18-labeling rates of phosphometabolites, critical in biochemical energy conversion and metabolic flux transmission. The biological usefulness of the J-decoupled P-31 NMR 2D chemical shift correlation maps was validated on adenosine tri-phosphate fractionally O-18 labeled in perfused mammalian hearts.
URI
https://doi.org/10.1007/s10858-011-9515-3
http://hdl.handle.net/11655/20135
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  • Temel Eczacılık Bilimleri Bölümü Makale Koleksiyonu [123]
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