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Highly Selective Magnetic Affinity Purification Of Histidine-Tagged Proteins By Ni2+ Carrying Monodisperse Composite Microspheres

Salimi, Kouroush; Usta, Duygu Deniz; Kocer, Ilkay; Celik, Eda; Tuncel, Ali (Royal Soc Chemistry, 2017)

A magnetic sorbent with stable and superior magnetic behaviour was developed for His-tagged protein purification by immobilized metal affinity chromatography (IMAC). Magnetic, monodisperse and porous silica microspheres 6 ...